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1 IL-10 haplotypes and TNF-α levels are associated with low muscle mass in patients with chronic hepatitis C
  1. Pedro Alves Soares Vaz de Castro1,
  2. Thais Pontelo de Vries1,2,
  3. Anna Luiza Soares Chagas1,
  4. Diego Alves Vieira1,
  5. Tatiana Bering1,2,
  6. Gifone Aguiar Rocha3 and
  7. Luciana Diniz Silva1,2,4
  1. ¹Ambulatório de Hepatites Virais do Instituto Alfa de Gastroenterologia do Hospital das Clínicas da Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil
  2. 2Programa de Pós-Graduação em Ciências Aplicadas à Saúde do Adulto da Faculdade Medicina da UFMG, Belo Horizonte, Brazil
  3. 3Laboratório de Pesquisa em Bacteriologia da Faculdade Medicina da UFMG, Belo Horizonte, Brazil
  4. 4Departamento de Clínica Médica, da Faculdade de Medicina da UFMG, Belo Horizonte, Brazil

Abstract

Background Despite the negative impact of low muscle mass (MM) on the survival of cirrhotic patients, the mechanisms linked to MM loss are not completely understood in patients with chronic hepatitis C (CHC).

Objectives To evaluate whether the IL-10 haplotype (-1082G>A, -819C>T, and -592C>A) and serum levels of tumour necrosis factor-alpha (TNF-α) were associated with low MM in CHC patients.

Methods 94 consecutive CHC outpatients (mean age, 50.3±11.5 yrs.; 74.5% males; 68.1% without cirrhosis and 31.9% with compensated cirrhosis) and 164 healthy controls were prospectively enrolled. SNPs were genotyped by RT-PCR. Serum levels of TNF-α were measured by ELISA. CHC patients, prospectively, underwent scanning of the lean tissue, appendicular skeletal muscle mass (ASM), and fat mass by dual-energy X-ray absorptiometry. The data analysed included appendicular skeletal mass (ASM) standardized for height (ASMI=ASM/height2). The cut-off points for low ASMI were 5.45 kg/m2 and 7.26 kg/m2 for women and men, respectively, according to Baumgartner et al. (1998). The International Physical Activity Questionnaire was used to determine the physical activity level.

Results IL-10 SNPs were in Hardy Weinberg equilibrium. Patients and healthy subjects showed the same distribution of genotypes. Low ASMI was found in 12/94 (12.8%) of the patients with CHC. The IL-10 haplotype ATA (low-producer genotype) was observed in 11/12 (91.7%) of the patients with low ASMI (P=0.03) and in only one of the patients without low ASMI 1/82 (1.2%) (Figure 1). In the multivariate analysis, low ASMI was significantly and independently associated with moderate-to-high physical activity (OR=0.31; 95%CI=0.09-0.98; P=0.05), TNF-α levels (OR=1.06; 95%CI=1.01-1.11; P=0.02) and ATA haplotype (OR=9.87; 95%CI=1.13-94.85; P=0.05).

Abstract 1 Figure 1
Abstract 1 Figure 1

Association between the prevalence of the IL-10 haplotype ATA and the low appendicular skeletal mass standardized for height (ASMI) in patients with chronic hepatitis C (CHC)

Conclusion This is the first study to demonstrate that the IL10 haplotype is associated with low ASMI in CHC patients. We also demonstrated that TNF-α is associated with low ASMI in CHC patients.

http://creativecommons.org/licenses/by-nc/4.0/

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

https://doi.org/10.1136/bmjnph-2022-nnedprosummit.8

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